fulltext.study @t Gmail

Quantification of methanogenic heterodisulfide reductase activity in biogas sludge

Paper ID Volume ID Publish Year Pages File Format Full-Text
23220 43420 2014 4 PDF Available
Title
Quantification of methanogenic heterodisulfide reductase activity in biogas sludge
Abstract

•Measurement of the activity of methanogens in biogas sludge by analysis of a key enzyme.•Development of a rapid test for quantification of heterodisulfide reductase in biogas sludge.•Quantification of heterodisulfide reductase in hydrogenotrophic and aceticlastic methanogens.

Methanogenic archaea are essential for the production of methane in biogas plants. Here we present enzymatic test systems for the analysis of the metabolic activity of methanogens based on the heterodisulfide reductase reaction. The first rapid test shows that heterodisulfide reductase can be detected in 1 g of biogas sludge after sonication and centrifugation. The resulting cell lysate used reduced methylviologen for heterodisulfide reduction, a reaction that is specifically catalyzed by methanogenic heterodisulfide reductase. In the second test cell lysate from 60 g of biogas sludge was separated by ultracentrifugation. Both, cytoplasmic membrane and cytoplasmic fractions revealed heterodisulfide reductase activity, indicating the presence of hydrogenotrophic and aceticlastic methanogens, respectively.

Keywords
BV, benzylviologen; BVred, reduced BV; CoM-S-S-CoB, disulfide from HS-CoM and HS-CoB; HDR, heterodisulfide reductase; HS-CoB, N-7-mercaptoheptanoyl-l-threonine phosphate; HS-CoM, 2-mercaptoethanesulfonate; MV, methylviologen; MVred, reduced MVBiogas plant
First Page Preview
Quantification of methanogenic heterodisulfide reductase activity in biogas sludge
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 180, 20 June 2014, Pages 66–69
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering