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Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy

Paper ID Volume ID Publish Year Pages File Format Full-Text
23226 43421 2014 10 PDF Available
Title
Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy
Abstract

•Efficient porcine circovirus Cap protein production.•Fuzzy logical DO control to automatically regulate methanol/sorbitol feeding.•Improving foreign protein production by P. pastoris with strong AOX promoter.•Restricting methanol consumption at moderately high level.•Stabilizing DO control under mild air-aeration condition.

Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the difference of DO change patterns in response to methanol and sorbitol additions, a novel fuzzy control system was proposed to automatically regulate the co-feeding rates of methanol and sorbitol for efficient Cap protein induction. With aid of the proposed control system when setting DO control level at 10%, overall fermentation performance was significantly improved: (1) DO could be stably controlled under mild aeration condition; (2) methanol consumption rate could be restricted at moderate level and the major enzymes involved with methanol metabolism were largely activated; (3) Cap protein concentration reached a highest level of 198 mg/L, which was about 64% increase over the best one using the pure methanol induction strategies.

Keywords
Cap protein; DO; Fermentation; Fuzzy control; Metabolic analysis
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Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 177, 10 May 2014, Pages 35–44
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us