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Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange

Paper ID Volume ID Publish Year Pages File Format Full-Text
23248 43424 2014 5 PDF Available
Title
Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange
Abstract

A flexible Sf9 insect cell line was recently developed leveraging the recombinase-mediated cassette exchange (RMCE) technology, which competes with the popular baculovirus expression vector system (BEVS) in terms of speed to produce new proteins. Herein, the ability of this cell platform to produce complex proteins, such as rotavirus core-like particles, was evaluated. A gene construct coding for a VP2-GFP fusion protein was targeted to a pre-characterized high recombination efficiency locus flanked by flipase (Flp) recognition target sites and, after three weeks in selection, an isogenic cell population was obtained. Despite the lower cell specific productivities with respect to those obtained by baculovirus infection, the titers of VP2-GFP reached in shake flask batch cultures were comparable as a result of higher cell densities. To further improve the VP2-GFP levels from stable expression, analysis of exhausted medium was undertaken to design feeding strategies enabling higher cell densities as well as increased culture duration. The implementation of the best strategy allowed reaching 20 million cells per ml in bioreactor cultures; the integrity of the rotavirus core-like particles could be confirmed by electron microscopy. Overall, we show that this Sf9-Flp cell platform represents a valuable alternative to the BEVS for producing complex recombinant proteins, such as rotavirus core-like particles.

Keywords
Flp-mediated site-specific integration; Virus-free insect cell expression; BEVS; Rotavirus core-like particles; Rational process optimization
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Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 171, 10 February 2014, Pages 34–38
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us