fulltext.study @t Gmail

Stereoselective synthesis of (R)-phenylephrine using recombinant Escherichia coli cells expressing a novel short-chain dehydrogenase/reductase gene from Serratia marcescens BCRC 10948

Paper ID Volume ID Publish Year Pages File Format Full-Text
23264 43426 2014 4 PDF Available
Title
Stereoselective synthesis of (R)-phenylephrine using recombinant Escherichia coli cells expressing a novel short-chain dehydrogenase/reductase gene from Serratia marcescens BCRC 10948
Abstract

•A short-chain dehydrogenase/reductase (SM_SDR) from Serratia marcescens.•SM_SDR prefers NADPH as cofactor.•E. coli BL21 (DE3) expressing SM_SDR can produce (R)-PE from HPMAE.•Fructose is the most effective carbon source for biocatalysis.

(R)-Phenylephrine [(R)-PE] is an α1-adrenergic receptor agonist and is widely used as a nasal decongestant to treat the common cold without the side effects of other ephedrine adrenergic drugs. We identified a short-chain dehydrogenase/reductase (SM_SDR) from Serratia marcescens BCRC 10948 that was able to convert 1-(3-hydroxyphenyl)-2-(methylamino) ethanone (HPMAE) into (R)-PE. The SM_SDR used NADPH and NADH as cofactors with specific activities of 17.35 ± 0.71 and 5.57 ± 0.07 mU/mg protein, respectively, at 30 °C and pH 7.0, thereby indicating that this enzyme could be categorized as an NADPH-preferring short-chain dehydrogenase/reductase. Escherichia coli strain BL21 (DE3) expressing SM_SDR could convert HPMAE into (R)-PE with more than 99% enantiomeric excess. The productivity and conversion yield were 0.57 mmol PE/l h and 51.06%, respectively, using 10 mM HPMAE. Fructose was the most effective carbon source for the conversion of HPMAE to (R)-PE.

Keywords
ADH, alcohol dehydrogenase; AKR, aldo-keto reductase; DDBDH, 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase; DHMPR, dihydromonapterin reductase; Dro_ADH, D. melanogaster alcohol dehydrogenase; GDH, glucose 1-dehydrogenase B; HBDH, d-beta-hydroxybutyrate
First Page Preview
Stereoselective synthesis of (R)-phenylephrine using recombinant Escherichia coli cells expressing a novel short-chain dehydrogenase/reductase gene from Serratia marcescens BCRC 10948
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 170, 20 January 2014, Pages 6–9
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us