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Hepatic differentiation of human embryonic stem cells on microcarriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
23277 43429 2014 10 PDF Available
Title
Hepatic differentiation of human embryonic stem cells on microcarriers
Abstract

•We employed microcarrier culture for differentiation of hESCs to hepatic cells.•hESCs differentiated in static culture and small-scale stirred bioreactor culture.•It showed comparable levels of hepatic differentiation to conventional 2D method.•The cells expressed hepatic lineage genes, proteins and functional characteristics.•Microcarriers will allow large-scale differentiation of hESCs to hepatic cells.

Translation of stem cell research to industrial and clinical settings mostly requires large quantities of cells, especially those involving large organs such as the liver. A scalable reactor system is desirable to ensure a reliable supply of sufficient quantities of differentiated cells. To increase the culture efficiency in bioreactor system, high surface to volume ratio needs to be achieved. We employed a microcarrier culture system for the expansion of undifferentiated human embryonic stem cells (hESCs) as well as for directed differentiation of these cells to hepatocyte-like cells. Cells in single cell suspension were attached to the bead surface in even distribution and were expanded to 1 × 106 cells/ml within 2 days of hESC culture with maintenance of the level of pluripotency markers. Directed differentiation into hepatocyte-like cells on microcarriers, both in static culture and stirred bioreactors, induced similar levels of hepatocyte-like cell differentiation as observed with cells cultured in conventional tissue culture plates. The cells expressed both immature and mature hepatocyte-lineage genes and proteins such as asialoglycoprotein receptor-1 (ASGPR-1) and albumin. Differentiated cells exhibited functional characteristics such as secretion of albumin and urea, and CYP3A4 activity could be detected. Microcarriers thus offer the potential for large-scale expansion and differentiation of hESCs induced hepatocyte-like cells in a more controllable bioreactor environment.

Keywords
Human embryonic stem cells; Microcarrier; Hepatic differentiation; Bioreactor; Suspension culture system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 174, 20 March 2014, Pages 39–48
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us