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Heterologous production of spectinomycin in Streptomyces venezuelae by exploiting the dTDP-d-desosamine pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
23278 43429 2014 7 PDF Available
Title
Heterologous production of spectinomycin in Streptomyces venezuelae by exploiting the dTDP-d-desosamine pathway
Abstract

Spectinomycin is an aminoglycoside antibiotic composed of actinamine and actinospectose, which are fused together by a putative glycosyltransferase, SpcG, during spectinomycin biosynthesis. Although previous studies have revealed the involvement of SpcA (myo-inositol monophosphatase), SpcB (dehydrogenase), SpcS2 (aminotransferase), and SpcM (methyltransferase) in the biosynthesis of actinamine, heterologous biosynthesis of spectinomycin via actinospectose has not been clearly elucidated. In this study, Streptomyces venezuelae was utilized as a source of dTDP-actinospectose from the pikromycin biosynthetic desosamine sugar pathway, and a recombinant vector, pSM5, carrying spcA, spcB, spcS2, spcM, and spcG was inserted into S. venezuelae. The formation of dTDP-spectinose was suspected through the use of dehydrogenase in the S. venezuelae chromosome. Herewith, the genetically engineered strain, S. venezuelae SM5, effectively produced up to 89.2 mg/L in optimized medium. However, pSM5 in S. venezuelae YJ003, a dTDP-actinospectose-deficient strain, did not produce spectinomycin. This result demonstrates the use of a dTDP-actinospectose precursor produced in the desosamine pathway for heterologous production of spectinomycin in S. venezuelae.

Keywords
Actinamine; Actinospectose; Desosamine pathway; Streptomyces venezuelae
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Heterologous production of spectinomycin in Streptomyces venezuelae by exploiting the dTDP-d-desosamine pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 174, 20 March 2014, Pages 57–63
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us