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Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
23284 43430 2013 6 PDF Available
Title
Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization
Abstract

•We identified one aldose 1-epimerase (Gal10) and two putative aldose 1-epimerases (Yhr210c and Ynr071c) in Saccharomyces cerevisiae.•Deletion of Gal10 significantly impairs the cellobiose utilization ability of engineered BY4741 strain.•AEP-deletion strains exhibit different mutarotase activities on cellobiose or glucose medium.•The endogenous regulation of AEP activities indicates a complicated system controlling cellobiose utilization may exist in S. cerevisiae.

Functional expression of a cellodextrin transporter and an intracellular β-glucosidase from Neurospora crassa in Saccharomyces cerevisiae enables simultaneous co-fermentation of cellobiose and non-glucose sugars such as xylose. Here we investigate the functional role of aldose 1-epimerase (AEP) in engineered cellobiose utilization. One AEP (Gal10) and two putative AEPs (Yhr210c and Ynr071c sharing 50.6% and 51.0% amino acid identity with Gal10, respectively) were selected. Deletion of GAL10 led to complete loss of both AEP activity and cell growth on cellobiose, while GAL10 complementation restored the AEP activity and cell growth. In addition, deletion of YHR210C or YNR071C resulted in improved cellobiose utilization. These results suggest that the intracellular mutarotation of β-glucose to α-glucose might be a rate controlling step and Gal10 play a crucial role in cellobiose fermentation by engineered S. cerevisiae.

Keywords
Cellobiose; β-Glucosidase; Aldose 1-epimerase; Cellulosic ethanol; Mutarotase
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Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 1, 10 October 2013, Pages 1–6
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us