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Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
23286 43430 2013 5 PDF Available
Title
Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis
Abstract

•Universal primer (SBEp) for spectral codification strategy via SBE reaction.•FRET based strategy for SNP identification in solution.•SBEp makes spectral codification a general strategy for SNP detection.•Validation of SBEp-spectral codification with clinical samples.

We previously reported a strategy that combines Förster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair.

Keywords
FRET; SBE; SNP; Universal SBE primer; Spectral codification
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Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 1, 10 October 2013, Pages 90–94
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us