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Chemical fragmentation for massively parallel sequencing library preparation

Paper ID Volume ID Publish Year Pages File Format Full-Text
23287 43430 2013 6 PDF Available
Title
Chemical fragmentation for massively parallel sequencing library preparation
Abstract

•Fragmentation is essential in most library preparation protocols.•A chemical fragmentation method has been adapted to high-throughput sequencing.•Chemical fragmentation resulted in high quality sequencing reads.•Chemical fragmentation is a cost-effective alternative other fragmentation methods.•Presented method has no observable sequence bias and requires no instrumentation.

Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron–EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.

Keywords
Fragmentation; High-throughput sequencing; Library preparation
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Chemical fragmentation for massively parallel sequencing library preparation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 1, 10 October 2013, Pages 95–100
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us