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Engineering Escherichia coli for improved ethanol production from gluconate

Paper ID Volume ID Publish Year Pages File Format Full-Text
23288 43430 2013 6 PDF Available
Title
Engineering Escherichia coli for improved ethanol production from gluconate
Abstract

•Deletion of pflA and ldh led to significant improvement of ethanol yield on gluconate.•Deletion of pflA and ldh led to small improvement of ethanol yield on glucose.•Deletion of pdh alone led to improved ethanol yield on gluconate.•Deletion of pdh alone led to no improvement of ethanol yield on glucose.

We report on engineering Escherichia coli to produce ethanol at high yield from gluconic acid (gluconate). Knocking out genes encoding for the competing pathways (l-lactate dehydrogenase and pyruvate formate lyase A) in E. coli KO11 eliminated lactate production, lowered the carbon flow toward acetate production, and improved the ethanol yield from 87.5% to 97.5% of the theoretical maximum, while the growth rate of the mutant strain was about 70% of the wild type. The corresponding genetic modifications led to a small improvement of ethanol yield from 101.5% to 106.0% on glucose. Deletion of the pyruvate dehydrogenase gene (pdh) alone improved the ethanol yield from 87.5% to 90.4% when gluconate was a substrate. The growth rate of the mutant strain was identical to that of the wild type. The corresponding genetic modification led to no improvements on ethanol yield on glucose.

Keywords
Escherichia coli; Gluconic acid; Ethanol; Pathway
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Engineering Escherichia coli for improved ethanol production from gluconate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 1, 10 October 2013, Pages 101–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us