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Propionic acid production in a plant fibrous-bed bioreactor with immobilized Propionibacterium freudenreichii CCTCC M207015

Paper ID Volume ID Publish Year Pages File Format Full-Text
23311 43431 2013 9 PDF Available
Title
Propionic acid production in a plant fibrous-bed bioreactor with immobilized Propionibacterium freudenreichii CCTCC M207015
Abstract

A plant fibrous-bed bioreactor (PFB) was constructed for propionic acid production. Sugar cane bagasse was applied to the PFB as immobilizing material. Starting at a concentration of 80 g/L of glucose, Propionibacterium freudenreichii CCTCC M207015 produced 41.20 ± 2.03 g/L of propionic acid at 108 h in the PFB. The value was 21.07% higher than that produced by free cell fermentation. Intermittent and constant fed-batch fermentations were performed in the PFB to optimize the fermentation results. The highest propionic acid concentration obtained from constant fed-batch fermentation was 136.23 ± 6.77 g/L, which is 1.40 times higher than the highest concentration (97.00 g/L) previously reported. Scanning electron microscopy analysis showed that cells exhibited striking changes in morphology after PFB domestication. Compared with free cell fermentation, the fluxes of propionic acid synthesis and the pentose phosphate pathway in PFB fermentation increased by 84.65% and 227.62%, respectively. On the other hand, a decrease in succinic and acetic acid fluxes was also observed. The metabolic flux distributions of the two PFB fed-batch fermentation strategies also demonstrated that constant fed-batch fermentation is a more beneficial method for the immobilized production of propionic acid. The relevant key enzyme activities and metabolic flux variations of the batch cultures showed good consistency. These results suggest that the PFB was effective in high-concentration propionic acid production.

► A plant fibrous-bed bioreactor was designed for propionic acid production. ► The highest propionic acid concentration of 136.23 g/L was obtained. ► Physiological changes, metabolic fluxes, and related enzyme activities were analyzed to elucidate the mechanism of PFB fermentation. ► PFB fermentation is very effective in high concentration propionic acid production.

Keywords
GLC, glucose; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde-3-phosphate; G3P, 3-phosphoglycerate; RU5P, ribulose-5-phosphate; CO2, carbon dioxide; XU5P, xylulose-5-phosphate; R5P, ribose-5-phosp
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Propionic acid production in a plant fibrous-bed bioreactor with immobilized Propionibacterium freudenreichii CCTCC M207015
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 164, Issue 2, March 2013, Pages 202–210
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us