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A novel method for preparing a protein-encapsulated bioaerogel: Using a red fluorescent protein as a model

Paper ID Volume ID Publish Year Pages File Format Full-Text
2332 108 2008 8 PDF Available
Title
A novel method for preparing a protein-encapsulated bioaerogel: Using a red fluorescent protein as a model
Abstract

A recombinant red fluorescent protein, DsRed, was chosen as a model protein to prepare a protein-encapsulated bioaerogel, DsRed-SAG. It was prepared using sol–gel polymerization of tetraethyl orthosilicate (TEOS) with an ionic liquid as the solvent and pore-forming agent. The DsRed-SAG bioaerogel was characterized by Fourier transformation infrared, scanning electron microscopy and Brunauer–Emmett-Teller measurements. It was found that the as-prepared bioaerogel had high porosity, and the silica network exhibited little shrinkage during the drying process. The stability of the bioaerogel was monitored by fluorescence spectroscopy and confirmed by confocal laser scanning microscopy. In addition, the protection of the encapsulated proteins by the silica network was further investigated using the degradation test by a protease. The results indicated that the as-prepared protein was quite stable during formation of the protein-containing wet gel and extraction of the ionic liquid, demonstrating that the new method can be extended to prepare other protein-encapsulated bioaerogels.

Keywords
Bioaerogel; DsRed; Ionic liquid; Protein-encapsulation
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A novel method for preparing a protein-encapsulated bioaerogel: Using a red fluorescent protein as a model
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 3, May 2008, Pages 725–732
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us