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A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue

Paper ID Volume ID Publish Year Pages File Format Full-Text
23323 43431 2013 6 PDF Available
Title
A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue
Abstract

Achondroplasia is the most common form of human dwarfism caused by a mutation in the fibroblast growth factor receptor 3 (FGFR3), resulting in abnormal endochondral bone formation. C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth and represents a potential therapy for achondroplasia. We have developed a novel, simple and cost effective method to produce a CNP analogue, PG-CNP37, at a large scale from Escherichia coli. A PG-CNP37 fusion protein was over-expressed as inclusion bodies in E. coli, which were purified then cleaved by formic acid to release the PG-CNP37 peptide. Approximately 0.5 g of 95% pure, soluble and active PG-CNP37 peptide was produced from 1 L of culture using this method and may represent a viable means for large-scale production of other therapeutic peptides.

► Novel method for producing an active 39-amino acid CNP analogue, PG-CNP37. ► Overexpression as inclusion bodies followed by formic acid cleavage. ► Achieved 80% pure PG-CNP37 peptide by a neutralization and centrifugation step. ► Final yield of 0.5 g/L PG-CNP37 peptide with 95% purity.

Keywords
CNP; E. coli; Fusion protein; Inclusion body; Formic acid cleavage; Large-scale
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A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 164, Issue 2, March 2013, Pages 196–201
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us