Enzymatic synthesis of c-di-GMP using inclusion bodies of Thermotoga maritima full-length diguanylate cyclase
Recombinant full-length diguanylate cyclases (DGCs) of Thermotoga maritima with native and mutant allosteric sites were overexpressed in Escherichia coli cells and characterized. It has been shown that target enzymes are produced substantially in the form of active inclusion bodies. Introduction of the mutation in allosteric site resulted in 7-fold increase of the T. maritima DGC activity. Possibility of applying full-length DGC of T. maritima in the form of inclusion bodies for synthesis of c-di-GMP was originally demonstrated.
► E. coli strains producing diguanylate cyclase of Thermotoga maritima were constructed. ► The obtained recombinant enzymes are produced in the form of active inclusion bodies. ► Diguanylate cyclase allosteric site inactivation resulted in increase of its activity. ► c-di-GMP was originally synthesized with the diguanylate cyclase inclusion bodies.
Journal: Journal of Biotechnology - Volume 164, Issue 2, March 2013, Pages 276–280