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Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha

Paper ID Volume ID Publish Year Pages File Format Full-Text
23349 43438 2013 9 PDF Available
Title
Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha
Abstract

Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. Hansenula polymorpha was engineered to catalyze the transformation of monomeric substrates to 5-hydroxyectoine. For this purpose four genes encoding the enzymes of the 5-hydroxyectoine biosynthesis pathway of Halomonas elongata, EctA, EctB, EctC, and EctD, were inserted into the genome of H. polymorpha. Subsequently the syntheses of ectoine and 5-hydroxyectoine were analyzed and optimized. We showed that H. polymorpha is a suitable system for recombinant 5-hydroxyectoine synthesis in gram per liter scale (2.8 g L−1 culture supernatant, 365 μmol/g dcw) in which almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity were achieved.

► Hansenula polymorpha was engineered to express the enzymes of the Halomonas elongata derived 5-hydroxyectoine biosynthesis pathway. ► Expression of EctB, EctA, EctC and EctD in H. polymorpha led to production of 5-hydroxyectoine in gram per liter scale. ► 5-Hydroxyectoine synthesis was influenced by feeding of methanol during cultivation.

Keywords
DABA, diaminobutyrate; EctA, DABA acyltransferase; EctB, DABA-2-oxoglutarate transaminase; EctC, ectoine synthase; EctD, ectoine hydroxylase; FMD, formate dehydrogenase; MOX, methanol oxidase; Ask, aspartate kinase; Asd, aspartate semialdehyde dehydrogena
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Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 167, Issue 2, 20 August 2013, Pages 85–93
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us