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Genome-wide identification of the targets for genetic manipulation to improve l-lactate production by Saccharomyces cerevisiae by using a single-gene deletion strain collection

Paper ID Volume ID Publish Year Pages File Format Full-Text
23368 43439 2013 9 PDF Available
Title
Genome-wide identification of the targets for genetic manipulation to improve l-lactate production by Saccharomyces cerevisiae by using a single-gene deletion strain collection
Abstract

•Gene manipulation targets to improve l-lactate production by yeast were identified.•For identification, a single-gene deletion strain collection was used.•Deletion of the genes related to ribosome biogenesis improved l-lactate production.•A single-gene deletion strain collection is useful to identify gene manipulation targets.

To identify genome-wide targets for gene manipulation for increasing l-lactate production in recombinant Saccharomyces cerevisiae strains, we transformed all available single-gene deletion strains of S. cerevisiae with a plasmid carrying the human l-lactate dehydrogenase gene, and examined l-lactate production in the obtained transformants. The thresholds of increased or decreased l-lactate production were determined based on l-lactate production by the standard strain in repetitive experiments. l-lactate production data for 4802 deletion strains were obtained, and deletion strains with increased or decreased l-lactate production were identified. Functional category analysis of genes whose deletion increased l-lactate production revealed that ribosome biogenesis-related genes were overrepresented. Most deletion strains for genes related to ribosome biogenesis exhibited increased l-lactate production in 200-ml batch cultures. We deleted the genes related to ribosome biogenesis in a recombinant strain of S. cerevisiae with a genetic background different from that of the above deletion strains, and examined the effect of target gene deletion on l-lactate production. We observed that deletion of genes related to ribosome biogenesis leads to increased l-lactate production by recombinant S. cerevisiae strains, and the single-gene deletion strain collection could be utilized in identifying target genes for improving l-lactate production in S. cerevisiae recombinant strains.

Keywords
Saccharomyces cerevisiae; l-Lactate; Single-gene deletion strain collection; Ribosome
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Genome-wide identification of the targets for genetic manipulation to improve l-lactate production by Saccharomyces cerevisiae by using a single-gene deletion strain collection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 2, 20 October 2013, Pages 185–193
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us