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Artificial niche substrates for embryonic and induced pluripotent stem cell cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
23373 43439 2013 11 PDF Available
Title
Artificial niche substrates for embryonic and induced pluripotent stem cell cultures
Abstract

•Recently used artificial niche substrates for embryonic and induced pluripotent stem cell culture in vitro are summarized.•Multicomponent and single component niche like substrates are discussed.•Multicomponent substrates include living feeder cells, chemically fixed feeder cells, and decellularized ECM scaffolds.•Single component substrates include ECM components, CAMs and growth factors.

Stem cells possess the ability to self-renew and differentiate into other cell types. In vivo, stem cells reside in their own anatomic niches in a defined physiological environment, from which they are released to differentiate into a required cell type when deemed appropriate. While a resident within the niche, the stem cell receives signals that in turn maintain the cell in a pluripotent state. In addition, the niche also provides nourishment to the cell. Physically, the niche also serves to anchor the cell via various ECM components and cell-adhesion molecules. Therefore, in vitro models that replicate the in vivo niche will lead to a better understanding of stem cell fate and turnover. In turn, this will help inform attempts to culture stem cells in vitro on artificial niche-like substrates. In this review, we have highlighted recent studies describing artificial niche-like substrates used to culture embryonic and induced pluripotent stem cells in vitro.

Keywords
Embryonic stem cell; Induced pluripotent stem cell; Niche; Pluripotency; Differentiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 168, Issue 2, 20 October 2013, Pages 218–228
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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