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Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
23491 43443 2013 5 PDF Available
Title
Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering
Abstract

•Transfer of the upstream pathway for 2,4,5-T to 2,5-DCHQ into BHC-A.•Engineered strain BHC-Ae degraded 2,4,5-T to 2,5-DCHQ.•BHC-Ae mineralized 2,5-DCHQ through γ-HCH downstream degradation pathway.

The gene cassette encoding for TftAB and TftCD proteins was integrated into the 16srDNA gene of the γ-hexachlorocyclohexane (γ-HCH) mineralizing strain Sphingobium sp. BHC-A by homologous recombination. The recombinant γ-HCH mineralizing strain may degrade 2,4,5-trichlorophenoxyacetic acid at a rate of 250 nmol mg [protein]−1 h−1, and the generated intermediate 2,5-dichlorohydroquinone may be further mineralized through γ-HCH downstream degradation pathway.

Keywords
γ-Hexachlorocyclohexane; 2,4,5-Trichlorophenoxyacetic acid; 2,5-Dichlorohydroquinone; Homologous recombination; Mineralization
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Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 166, Issue 4, 20 July 2013, Pages 187–191
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us