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Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate

Paper ID Volume ID Publish Year Pages File Format Full-Text
23500 43444 2013 7 PDF Available
Title
Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate
Abstract

•Pseudomonas fluorescens pools pyruvate upon H2O2 stress.•No genetic modification is required to trigger pyruvate formation.•NADPH production is up-regulated, while NADH levels are curtailed.•Metabolomic and proteomic assays are used to ascertain these findings.•A cost effective green alternative to pyruvate-producing technologies.

Pseudomonas fluorescens invoked a metabolic reconfiguration that resulted in enhanced production of pyruvate under the challenge of hydrogen peroxide (H2O2). Although this stress led to a sharp reduction in the activities of numerous tricarboxylic acid (TCA) cycle enzymes, there was a marked increase in the activities of catalase and various NADPH-generating enzymes to counter the oxidative burden. The upregulation of phosphoenolpyruvate synthase (PEPS) and pyruvate kinase (PK) coupled with the reduction of pyruvate dehydrogenase (PDH) in the H2O2-challenged cells appear to be important contributors to the elevated levels of pyruvate found in these bacteria. Increased pyruvate synthesis was evident in the presence of a variety of carbon sources including d-glucose. Intact cells rapidly consumed d-glucose with the concomitant formation of this monocarboxylic acid. At least a 12-fold increase in pyruvate production within 1 h was observed in the stressed cells. These findings may be exploited in the development of technologies aimed at the conversion of carbohydrates into pyruvate.

Keywords
6PGDH, 6-phosphogluconate dehydrogenase; BN-PAGE, blue native polyacrylamide gel electrophoresis; BSA, bovine serum albumin; CFE, cell free extract; ETC, electron transport chain; FUM, fumarase; GDH, glutamate dehydrogenase; G6PDH, glucose-6-phosphate deh
First Page Preview
Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 167, Issue 3, 10 September 2013, Pages 309–315
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering