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Mathematical model for aldol addition catalyzed by two d-fructose-6-phosphate aldolases variants overexpressed in E. coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
23502 43444 2013 10 PDF Available
Title
Mathematical model for aldol addition catalyzed by two d-fructose-6-phosphate aldolases variants overexpressed in E. coli
Abstract

•Kinetic model for aldol addition catalyzed by two d-fructose-6-phosphate aldolase variants.•The same type of kinetic model could be applied for both enzymes.•Operational stability of enzymes was assessed in the batch and ultrafiltration membrane reactor.

Two d-fructose-6-phosphate aldolase variants namely, single variant FSA A129S and double variant FSA A129S/A165G, were used as catalysts in the aldol addition of dihydroxyacetone (DHA) to N-Cbz-3-aminopropanal. Mathematical model for reaction catalyzed by both enzymes, consisting of kinetic and mass balance equations, was developed. Kinetic parameters were estimated from the experimental data gathered by using the initial reaction rate method. The model was validated in the batch and continuously operated ultrafiltration membrane reactor (UFMR). The same type of kinetic model could be applied for both enzymes. The operational stability of the aldolases was assessed by measuring enzyme activity during the experiments. FSA A129S/A165G had better operational stability in the batch reactor (half-life time 26.7 h) in comparison to FSA A129S (half-life time 5.78 h). Both variants were unstable in the continuously operated UFMR in which half-life times were 1.99 and 3.64 h for FSA A129S and FSA A129S/A165G, respectively.

Keywords
d-Fructose-6-phosphate aldolase; Enzyme kinetics; Mathematical model
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Mathematical model for aldol addition catalyzed by two d-fructose-6-phosphate aldolases variants overexpressed in E. coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 167, Issue 3, 10 September 2013, Pages 191–200
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us