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ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
23524 43446 2013 8 PDF Available
Title
ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae
Abstract

•PDR1 R821S mutant had tolerance to a hydrophilic organic solvent, as well as hydrophobic.•We identified ABC transporters and cell wall proteins involved in organic solvent tolerance.•The proteins involved in organic solvent tolerance varied according to solvent properties.•The improvement of tolerance to specific organic solvents would contribute to the practical use.

Pleiotropic drug resistance 1 (Pdr1p) protein is a key transcription factor in multidrug resistance. Specifically, R821S point mutation in the PDR1 gene in Saccharomyces cerevisiae diploid KK-211 strain plays an important role in tolerance to hydrophobic organic solvents, though the molecular mechanisms underlying organic solvent tolerance are unclear. In KK-211, several ABC transporters and cell wall proteins were upregulated. PDR1 R821S mutant derived from the laboratory haploid strain MT8-1 confirmatively tolerated hydrophobic organic solvents, and this study is the first to reveal its tolerance of the hydrophilic organic solvent, dimethyl sulfoxide (DMSO). To identify the genes involved in the organic solvent tolerance, we focused on the upregulated 4 ABC transporters and 6 cell wall proteins in KK-211, and demonstrated 2 ABC transporters, Pdr10p and Snq2p, and the 4 cell wall proteins, Wsc3p, Pry3p, Pir1p, and Ynl190wp were responsible for hydrophobic organic solvent (n-decane and n-undecane) tolerance. Tolerance to the hydrophilic organic solvent, DMSO, was facilitated by the overproduction of 2 ABC transporters, Snq2p and Yor1p and, 3 cell wall proteins, Wsc3p, Pry3p, and Ynl190wp. Our results suggest that overexpression of genes encoding proteins effective for tolerance to specific organic solvents would enable enhanced tolerances for practical use.

Keywords
ABC transporter; Cell wall protein; Organic solvent tolerance; Saccharomyces cerevisiae
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ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 2, 20 May 2013, Pages 145–152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us