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Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection

Paper ID Volume ID Publish Year Pages File Format Full-Text
23527 43446 2013 8 PDF Available
Title
Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection
Abstract

•Gaussia luciferase-lactadherin fusion protein was designed to label exosomes.•Exosomes were successfully labeled with the fusion protein.•In vivo pharmacokinetics of the exosomes can be visualized by this new technology.

The development of exosomes as delivery vehicles requires understanding how and where exogenously administered exosomes are distributed in vivo. In the present study, we designed a fusion protein consisting of Gaussia luciferase and a truncated lactadherin, gLuc-lactadherin, and constructed a plasmid expressing the fusion protein. B16-BL6 murine melanoma cells were transfected with the plasmid, and exosomes released from the cells were collected by ultracentrifugation. Strong luciferase activity was detected in the fraction containing exosomes, indicating their efficient labeling with gLuc-lactadherin. Then, the labeled B16-BL6 exosomes were intravenously injected into mice, and their tissue distribution was evaluated. Pharmacokinetic analysis of the exosome blood concentration–time profile revealed that B16-BL6 exosomes disappeared very quickly from the blood circulation with a half-life of approximately 2 min. Little luciferase activity was detected in the serum at 4 h after exosome injection, suggesting rapid clearance of B16-BL6 exosomes in vivo. Moreover, sequential in vivo imaging revealed that the B16-BL6 exosome-derived signals distributed first to the liver and then to the lungs. These results indicate that gLuc-lactadherin labeling is useful for tracing exosomes in vivo and that B16-BL6 exosomes are rapidly cleared from the blood circulation after systemic administration.

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Keywords
Exosome; Gaussia luciferase; Lactadherin; Biodistribution
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Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 2, 20 May 2013, Pages 77–84
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us