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Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants

Paper ID Volume ID Publish Year Pages File Format Full-Text
23529 43446 2013 6 PDF Available
Title
Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants
Abstract

•We examined the influence of the gold and silver nanoparticles on type and frequency of flax calli regeneration.•We detected the gold and silver nanoparticles in flax calli and regenerants.•We demonstrate a method for direct delivery of metal nanoparticles to plant cells by vacuum deposition of metal nanolayers on powdered phytohormone.

We demonstrate a method for direct delivery of metal nanoparticles to flax calli and regenerant cells by vacuum deposition of metal nanolayers on powdered hormone followed by dispersal of the combined hormone-metal in medium. The penetration and location of the gold (AuNPs) and silver (AgNPs) nanoparticles in calli and in plant regenerants were confirmed by optical absorption spectroscopy and scanning electron microscopy. We detected a significant effect of the AuNPs and AgNPs on the regeneration type of flax calli.

Keywords
Biotechnology; Silver and gold nanoparticles; Calli culture; Flax; Scanning electron microscopy
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Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 2, 20 May 2013, Pages 127–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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