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Surface elasticity and charge concentration-dependent endothelial cell attachment to copolymer polyelectrolyte hydrogel

Paper ID Volume ID Publish Year Pages File Format Full-Text
2354 109 2009 8 PDF Available
Title
Surface elasticity and charge concentration-dependent endothelial cell attachment to copolymer polyelectrolyte hydrogel
Abstract

The surface micromechanical properties of 2-hydroxyethyl methacrylate (HEMA) and 2-methacryloxyethyl trimethyl ammonium chloride (MAETAC) copolymer hydrogels are probed using atomic force microscopy. HEMA–MAETAC polyelectrolyte hydrogels with increasing positive charge concentrations ranging from 0 to 400 mM in increments of 40 mM, are fabricated using different proportions of HEMA and MAETAC monomers. Increasing proportions of positively charged MAETAC monomers produce hydrogels with increasingly swollen states and correspondingly decreasing measures of stiffness, or Young’s modulus. Increasing the relative proportion of charged monomers also increases the hysteresis in the approaching and retracting components of the force spectroscopy curves. When these hydrogels are equilibrated in cell-culture media without fetal bovine serum and a pH-controlled CO2 environment, precipitation reactions increase the variability of the Young’s modulus estimates. Adding a buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, maintains physiological pH without the use of a CO2 environment, and thus reduces salt precipitation reactions and the variability of the Young’s modulus. The attachment of porcine pulmonary artery endothelial cells increases with increasing prepared hydrogel charge concentration and decreasing elasticity.

Keywords
Hydrogel; Positive charge concentration; AFM (atomic force microscopy); Elasticity; Swelling
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Surface elasticity and charge concentration-dependent endothelial cell attachment to copolymer polyelectrolyte hydrogel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 1, January 2009, Pages 144–151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us