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Complete conversion of major protopanaxadiol ginsenosides to compound K by the combined use of α-l-arabinofuranosidase and β-galactosidase from Caldicellulosiruptor saccharolyticus and β-glucosidase from Sulfolobus acidocaldarius

Paper ID Volume ID Publish Year Pages File Format Full-Text
23547 43451 2013 8 PDF Available
Title
Complete conversion of major protopanaxadiol ginsenosides to compound K by the combined use of α-l-arabinofuranosidase and β-galactosidase from Caldicellulosiruptor saccharolyticus and β-glucosidase from Sulfolobus acidocaldarius
Abstract

•α-l-Arabinofuranosidase and/or β-galactosidase, and β-glucosidase were combined.•The conditions for compound K production of the enzymes were optimized.•The enzymes completely converted major protopanaxadiol ginsenosides to compound K.

The ginsenoside compound K has pharmaceutical activities, including anti-tumor, anti-inflammatory, anti-allergic, and hepatoprotective effects. To increase the production of compound K, the α-l-arabinofuranoside-hydrolyzing α-l-arabinofuranosidase (CS-abf) and/or the α-l-arabinopyranoside-hydrolyzing β-galactosidase from Caldicellulosiruptor saccharolyticus (CS-bgal) were mixed with the β-d-glucopyranoside-hydrolyzing β-glucosidase from Sulfolobus acidocaldarius (SA-bglu). The optimum conditions for the production of ginsenoside compound K from ginsenoside Rc or Rb2, or from major protopanaxadiol ginsenosides in ginseng root extract were determined to be pH 6.0 and 75 °C with 8 mg ml−1 ginsenoside Rc, 8 mg ml−1 Rb2, or 10% (w/v) ginseng root extract; and 10.5 U ml−1 CS-abf or CS-bgal supplemented with 4.5 U ml−1 SA-bglu, or 10.5 U ml−1 CS-abf and 10.5 U ml−1 CS-bgal supplemented with 4.5 U ml−1 SA-bglu, respectively. Under optimum conditions, ginsenosides Rc and Rb2, and major protopanaxadiol ginsenosides in ginseng root extract were completely converted to compound K after 12, 14, and 20 h, respectively, with the respective productivities of 388, 328, and 144 mg l−1 h−1. This is the first report of the complete conversion of major protopanaxadiol ginsenosides to compound K.

Keywords
Compound K; α-l-Arabinofuranosidase; β-Galactosidase; β-Glucosidase; Caldicellulosiruptor saccharolyticus; Sulfolobus acidocaldarius
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Complete conversion of major protopanaxadiol ginsenosides to compound K by the combined use of α-l-arabinofuranosidase and β-galactosidase from Caldicellulosiruptor saccharolyticus and β-glucosidase from Sulfolobus acidocaldarius
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 167, Issue 1, 10 August 2013, Pages 33–40
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us