fulltext.study @t Gmail

Genome-wide overexpression screen for sodium acetate resistance in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
23557 43452 2013 8 PDF Available
Title
Genome-wide overexpression screen for sodium acetate resistance in Saccharomyces cerevisiae
Abstract

The production of biofuels from cellulosic biomass is a promising technology for developing a renewable source of energy. Efforts to produce ethanol from cellulosic biomass using microbes, such as the yeast Saccharomyces cerevisiae, face major challenges, including the need for detoxification. Here, we apply a strategy to discover genetic alterations that lead to improved robustness of S. cerevisiae in the presence of acetate, which is present at toxic concentrations in hemicellulose hydrolysates. Acetate in its protonated form (acetic acid) enters the cell through passive diffusion and dissociates into a proton and acetate, acidifying the cytosol and inhibiting cell function, an effect that is exacerbated in the presence of sodium. Through flow cytometry analysis, implemented as part of a novel cell culture technique, the Cytostat, we characterized the deleterious effects of sodium acetate on growth and on cell size homeostasis. Further, using the Cytostat to screen a genome-wide, gene overexpression library, we identified that overexpressing the ENA2 gene, a P-type sodium pump ATPase, provides a significant growth improvement in the presence of sodium acetate. Together, our data support the proposed mechanism for the synergistic growth inhibition exerted by acetate and sodium, as well as the mechanism that develops resistance.

► The Cytostat isolated sodium acetate resistant clones from overexpression library. ► ENA2 sodium pump overexpression improves growth under sodium acetate stress. ► Sodium strongly exacerbates the deleterious effect of acetic acid on Saccharomyces cerevisiae. ► Mechanism of resistance consists of coordinated transport of ions across membrane.

Keywords
Flow cytometry; Cytostat; Overexpression library; Sodium acetate; cell size
First Page Preview
Genome-wide overexpression screen for sodium acetate resistance in Saccharomyces cerevisiae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 164, Issue 1, 10 March 2013, Pages 26–33
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us