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Application of memberane dispersion for enhanced lipid milking from Botryococcus braunii FACHB 357

Paper ID Volume ID Publish Year Pages File Format Full-Text
23579 43453 2013 8 PDF Available
Title
Application of memberane dispersion for enhanced lipid milking from Botryococcus braunii FACHB 357
Abstract

To improve the mixing efficiency in an aqueous–tetradecane system and thus to increase the lipid milking efficiency, poly (ether sulfones) hollow fiber membrane was applied as dispersion medium to establish an in situ lipid extraction process from Botryococcus braunii FACHB 357. The lipid location of this microalga was characterized by fluorescence microscope and transmission electron microscopy, respectively. The results showed that B. braunii excreted lipids into the outer matrix, which allowed it possible to extract algal lipids in situ by organic solvent. Within an aqueous–organic biphasic system, the lipid extraction ratio of tetradecane increased from 38.05% to 50.15% by introducing a microporous membrane as the dispersion medium, mainly because smaller solvent droplets were produced. Under this experimental condition (the volume ratio of tetradecane: 10%, the flow rate: 10 ml min−1), solvent toxicity and shearing stress had not shown significant impact on algal cells viability in 96 h. Within the same time period, the lipid amount extracted by solvent was enhanced with the increase of the solvent flow rate and the initial biomass concentration. These results suggested membrane dispersion was a good choice to improve mixing effect in the algal lipid milking process or other similar cell products extracted processes.

► An in situ algal lipid extraction process was established for lipids recovery from Botryococcus braunii FACHB 357 by the biocompatible tetradecane solvent. ► Hollow fiber micro-filtration membrane was used as dispersion medium to enhance the mixing efficiency and the algal lipid extractability in a biphasic system. ► 92% of algal cells were found still viable within the aqueous–tetradecane system after 96 h treatment.

Keywords
Microalgae; Botryococcus braunii; Lipid extraction; Membrane dispersion; Biphasic system
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Application of memberane dispersion for enhanced lipid milking from Botryococcus braunii FACHB 357
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 1, 10 May 2013, Pages 22–29
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us