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Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste

Paper ID Volume ID Publish Year Pages File Format Full-Text
23581 43453 2013 7 PDF Available
Title
Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste
Abstract

A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (μmax.) of 0.08 h−1, 0.10 h−1 and 0.13 h−1, respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Pseudomonas chlororaphis was investigated for production of mcl-PHA latex under controlled conditions on bioreactor scale ► A surplus material, namely saturated biodiesel fraction from animal waste lipids, was used as the sole carbon source ► A detailed kinetic analysis of the bioprocess is provided ► Productivity of the mcl-PHA latex is competitive to other mcl-PHA producing organisms on expensive carbon sources.

Keywords
Biodegradable resin; Biodiesel; mcl-PHA; Polyhydroxyalkanoate; Pseudomonas chlororaphis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 1, 10 May 2013, Pages 45–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us