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Exploring the synthetic potential of cell bound β-glycosidase of Pichia etchellsii

Paper ID Volume ID Publish Year Pages File Format Full-Text
23583 43453 2013 6 PDF Available
Title
Exploring the synthetic potential of cell bound β-glycosidase of Pichia etchellsii
Abstract

Enzymatic synthesis of oligosaccharides with absolute stereo-selectivity and regio-selectivity provides an economical alternative to classical chemical methods. Here we demonstrate, for the first time, that whole cells of P. etchellsii are highly efficient biocatalysts and can be used for oligosaccharide synthesis using p-nitrophenyl-β-d-glucopyranoside, o-nitrophenyl-β-d-glucopyranoside and p-nitrophenyl-β-d-xylopyranoside as both donors and acceptors. Auto-condensation of p-nitrophenyl-β-d-glucopyranoside and o-nitrophenyl-β-d-glucopyranoside resulted in formation of β-(1 → 6) linked disaccharide as major products in 4 and 12% yield respectively. By contrast, auto condensation of p-nitrophenyl-β-d-xylopyranoside exclusively lead to formation of β-(1 → 4) linked disaccharide in 24% yield.

► Enzymatic synthesis of aryl-oligosaccharides using Pichia etchellsii whole cells as biocatalysts. ► Absolute stereo-selectivity and regio-selectivity using the whole cell biocatalyst approach in buffer. ► Auto-condensation of p-nitrophenyl-β-d-glucopyranoside leading to formation of a β-(1 → 6) linked disaccharide in 4% yield. ► Auto-condensation of o-nitrophenyl-β-d-glucopyranoside resulting in formation of a β-(1 → 6) linked disaccharide in 12% yield. ► Auto condensation of p-nitrophenyl-β-d-xylopyranoside resulting in formation of β-(1 → 4) linked disaccharide in 24% yield.

Keywords
Auto-condensation; Pichia etchellsii; Transglycosylation; β-glycosidaseOG, octyl-β-d-glucopyranoside; pNPβG, p-nitrophenyl-β-d-glucopyranoside; MG, methyl-β-d-glucopyranoside; pNPβX2, p-nitrophenyl-β-d-xylopyranosyl-1 → 4-β-d-xylopyranoside; pNPβGal, p-ni
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 165, Issue 1, 10 May 2013, Pages 63–68
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us