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Expression of wheat expansin driven by the RD29 promoter in tobacco confers water-stress tolerance without impacting growth and development

Paper ID Volume ID Publish Year Pages File Format Full-Text
23600 43456 2013 11 PDF Available
Title
Expression of wheat expansin driven by the RD29 promoter in tobacco confers water-stress tolerance without impacting growth and development
Abstract

Expansins are the key regulators of cell wall extension during plant growth. Previously, we produced transgenic tobacco plants with increased tolerance to water stress by overexpressing the wheat expansin gene TaEXPB23 driven by the constitutive 35S cauliflower mosaic virus (CaMV) promoter. However, the growth and development of 35S::TaEXPB23 transgenic tobacco plants were altered under normal growth conditions, with a faster growth rate at the seedling stage, earlier flowering and maturation, and a shorter plant height compared to WT. In the current study, we determined that cellular characteristics and carbohydrate metabolism were altered in 35S::TaEXPB23 transgenic tobacco plants. We also generated transgenic Arabidopsis plants using the same vector. The transgenic Arabidopsis plants had the same phenotype as the transgenic tobacco plants, which may have resulted from the altered expression of several flowering-related genes. We then produced TaEXPB23 transgenic tobacco plants using the stress-inducible RD29A promoter. The use of this promoter reduced the negative effects of TaEXPB23 on plant growth and development. The RD29A::TaEXPB23 transgenic tobacco plants had greater tolerance to water stress than WT, as determined by examining physiological and biochemical parameters. Therefore, the use of stress-inducible promoters, such as RD29A, may minimize the negative effects of constitutive transgene expression and improve the water-stress tolerance of plants.

► Overexpressing transgenic tobacco plants exhibited improved drought tolerance. ► The development and phenotype of 35S::EXPB23 transgenic plants were severely retarded. ► Overexpressing transgenic Arabidopsis plants exhibited similarity phenotype. ► The RD29A::EXPB23 tobaccos showed improved phenotype and enhanced drought tolerance.

Keywords
DW, dry weight; Fv/Fm, maximal efficiency of PSII photochemistry; FW, fresh weight; Gs, leaf stomatal conductance; MS, Murashige-Skoog; MDA, malonyldialdehyde; Pn, net photosynthetic rate; PSII, photosystem II; ΦPSII, actual efficiency of PSII photochemis
First Page Preview
Expression of wheat expansin driven by the RD29 promoter in tobacco confers water-stress tolerance without impacting growth and development
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 3, 10 February 2013, Pages 281–291
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering