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Development of fed-batch strategies for the production of streptavidin by Streptomyces avidinii based on power input and oxygen supply studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
23604 43456 2013 8 PDF Available
Title
Development of fed-batch strategies for the production of streptavidin by Streptomyces avidinii based on power input and oxygen supply studies
Abstract

Streptavidin is a tetrameric protein with an extremely high affinity to biotin and different biotin-like peptide-tags. This characteristic causes its widespread use in biotechnology. Streptavidin is produced by the fermentation of wild type Streptomyces avidinii or by recombinant Streptomyces lavendulae, Escherichia coli, and Bacillus subtilis strains. However, little is known about the influence of power input and oxygen supply as well as feeding strategies on the production of streptavidin by S. avidinii. This paper provides a systematic analysis of the effect of rotary frequency of the stirrer, leading to a plateau-like streptavidin formation behaviour between 400 and 700 min−1. This plateau was characterized by specific power inputs between 79 and 107 W L−1 and corresponding maximal product concentrations of 6.90 μM in 6 days. Lower as well as higher rotary frequencies were not beneficial. Subsequently, a linear fed-batch procedure could be established reproducibly yielding 39.20 μM streptavidin in 14 days, characterized by a constant productivity of 114 nM h−1. Fed-batch procedures based on dissolved oxygen were less efficient. The linear feeding strategy presented in this paper led to the highest streptavidin concentration ever reported and exceeded the maximal product level given in the literature drastically by a factor of 8.5.

► We give an overview on microbial streptavidin production systems. ► We show the influence of shear stress and oxygen supply on S. avidinii concerning biomass and product formation. ► Glucose deprivation was accompanied by mycelial instability at high rotary frequencies of the stirrer. ► This results in the development of several fed-batch procedures. ► The maximal product level given in the literature was increased drastically by a factor of 8.5.

Keywords
SAV, streptavidinFed-batch cultivation; Oxygen supply; Power input; Shear stress; Streptavidin production; Streptomyces avidinii
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Development of fed-batch strategies for the production of streptavidin by Streptomyces avidinii based on power input and oxygen supply studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 3, 10 February 2013, Pages 325–332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us