fulltext.study @t Gmail

Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
23607 43456 2013 6 PDF Available
Title
Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice
Abstract

Rabies is one of the most fatal zoonotic diseases in developing countries, where a safe, cheap and effective vaccine against the disease remains unaffordable. In this paper, we describe a new silkworm-baculovirus expression system to express the nucleoprotein (N) gene of rabies virus and evaluation of the immune response in BALB/c mice. A recombinant baculovirus -rBmNPV(RV-N) carrying the N gene of rabies virus Evelyn Rokitniki Abelseth (ERA) strain was constructed and the N protein expression was evaluated in Bombyx mori (BmN) cells and silkworm pupae by immunofluorescence staining, Western blots and enzyme-linked immunosorbent assay (ELISA). The immune response to vaccines was evaluated based on serum IgG antibody titers and challenge experiments. The study revealed that N protein of rabies virus can be highly expressed in silkworm baculovirus expression system and the vaccine of N antigen presents a promising approach for the prevention of rabies virus.

► The rabies virus nucleoprotein was expressed in silkworm pupae. ► The yield of purified protein up to 50 mg from 1 L of crude pupae extracts. ► Mice were vaccinated with purified nucleoprotein can induce specific antibodies. ► The survival rates of mice immunized with N antigen showed 90% protection.

Keywords
Rabies virus; Nucleoprotein; Recombinant silkworm baculovirus; Vaccine
First Page Preview
Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 3, 10 February 2013, Pages 333–338
Authors
, , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us