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Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters

Paper ID Volume ID Publish Year Pages File Format Full-Text
23608 43456 2013 7 PDF Available
Title
Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters
Abstract

We have developed a novel bi-directional promoter (FsFfCBD) by placing two heterogeneous core-promoters from the Figwort mosaic virus sub-genomic transcript promoter (FsCP, −69 to +31) and Cauliflower mosaic virus 35S promoter (CCP, −89 to +1) respectively on upstream (5’) and downstream (3’) ends of a tri-hybrid enhancer (FsEFfECE), in reverse orientation. The FsEFfECE domain encompasses three heterologous enhancer fragments from Figwort mosaic virus sub-genomic transcript promoter (FsE, 101 bp, −70 to −170), Figwort mosaic virus full-length transcript promoter (FfE, 196 bp, −249 to −54) and Cauliflower mosaic virus 35S promoter (CE, 254 bp, −343 to −90). The bi-directional nature of the FsFfCBD promoter (coupled to GFP and GUS) was established both in transient systems (onion epidermal cells and tobacco protoplasts) and transgenic plant (Nicotiana tabacum samsun NN) by monitoring the simultaneous expression of GFP and GUS employing fluorescence (for GFP) and biochemical (for GUS) based assays. In transgenic plants, the FsFfCBD promoter was found to be 6.8 and 2.5 times stronger than two parent promoters; Fs and FfC respectively. The bi-directional compound promoter FsFfCBD, composed of three heterologous enhancers with enhanced activity could become a valuable additional tool for efficient plant metabolic engineering and molecular pharming.

► It contains tripartite enhancer domain. ► It showed enhanced activity. ► It is functional in onion and tobacco plants.

Keywords
Bi-directional promoter; CLSM; FMV; Tripartite enhancer; GFP; GUS
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Development of an efficient bi-directional promoter with tripartite enhancer employing three viral promoters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 3, 10 February 2013, Pages 311–317
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us