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On-line multiple component analysis for efficient quantitative bioprocess development

Paper ID Volume ID Publish Year Pages File Format Full-Text
23612 43457 2013 9 PDF Available
Title
On-line multiple component analysis for efficient quantitative bioprocess development
Abstract

On-line monitoring devices for the precise determination of a multitude of components are a prerequisite for fast bioprocess quantification. On-line measured values have to be checked for quality and consistency, in order to extract quantitative information from these data.In the present study we characterized a novel on-line sampling and analysis device comprising an automatic photometric robot. We connected this on-line device to a bioreactor and concomitantly measured six components (i.e. glucose, glycerol, ethanol, acetate, phosphate and ammonium) during different batch cultivations of Pichia pastoris. The on-line measured data did not show significant deviations from off-line taken samples and were consequently used for incremental rate and yield calculations. In this respect we highlighted the importance of data quality and discussed the phenomenon of error propagation. On-line calculated rates and yields depicted the physiological responses of the P. pastoris cells in unlimited and limited cultures. A more detailed analysis of the physiological state was possible by considering the off-line determined biomass dry weight and the calculation of specific rates.Here we present a novel device for on-line monitoring of bioprocesses, which ensures high data quality in real-time and therefore refers to a valuable tool for Process Analytical Technology (PAT).

► A novel on-line monitoring device is described. ► We show the high frequent precise determination of multiple components in Pichia pastoris cultivations. ► The data quality was demonstrated by extraction of information. ► Physiological responses of microbial cultures upon media limitations were discussed efficiently.

Keywords
BDW, biomass dry weight; CER, carbon dioxide evolution rate; DoE, degree of reduction; FDA, Food and Drug Administration; FIA, Flow Injection Analysis; HPLC, high performance liquid chromatography; ICH, International Conference on Harmonization; NH4, ammo
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 4, 20 February 2013, Pages 362–370
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us