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Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
23613 43457 2013 6 PDF Available
Title
Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
Abstract

A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.

► A biosensor chip with integrated microfluidic channel and enzyme-based sensors was developed. ► The chip was successfully used for measurement of glucose, glutamate and glutamine. ► Cost-effective and small-sized analysis tool with opportunity to integrate further sensors.

Keywords
Amperometric enzyme sensor; Biosensor chip; Glucose; Glutamate; Glutamine; Flow-injection analysis
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Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 163, Issue 4, 20 February 2013, Pages 371–376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us