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Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter

Paper ID Volume ID Publish Year Pages File Format Full-Text
23624 43459 2012 5 PDF Available
Title
Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter
Abstract

cis-Epoxysuccinate hydrolase (ESH) from Nocardia tartaricans CAS-52 could catalyze the stereospecific hydrolysis of cis-epoxysuccinate to l-(+)-tartrate. The ESH gene of 762 bp was cloned and its open reading frame (ORF) sequence predicted a protein of 253 amino acids. An expression plasmid carrying the ESH gene under the control of the PLPR promoter was introduced into Escherichia coli, and the ESH gene was successfully expressed in the recombinant strain. The expression conditions and scale-up production were also studied. Fed-batch fermentation of E. coli Trans 1-T1 transformant was carried out in a 2000 L fermentor to product recombinant ESH. The results showed that wet cell concentration reached to 62.45 g L−1, and the specific activity of ESH was 380.17 U mg−1, which could meet the requirements of industrial production of l-(+)-tartaric acid.

► An engineering bacterium is constructed to express recombinant ESH with PLPR promoter. ► The phage resistant strain is chosen to avoid phage pollution. ► Realizing the industrial production of recombinant ESH.

Keywords
cis-Epoxysuccinate hydrolase; PLPR promoter; Prokaryotic expression; Scale-up
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Expression and production of recombinant cis-epoxysuccinate hydrolase in Escherichia coli under the control of temperature-dependent promoter
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issues 2–3, 31 December 2012, Pages 232–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us