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Phage display allows identification of zona pellucida-binding peptides with species-specific properties: Novel approach for development of contraceptive vaccines for wildlife

Paper ID Volume ID Publish Year Pages File Format Full-Text
23629 43459 2012 8 PDF Available
Title
Phage display allows identification of zona pellucida-binding peptides with species-specific properties: Novel approach for development of contraceptive vaccines for wildlife
Abstract

Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific properties, an important feature for wildlife oral vaccines as the goal is to control overpopulation of a target species while not affecting non-target species’ reproduction. Six phage-peptide antigens were injected intramuscularly into pigs and corresponding immune responses evaluated. Administration of the antigens into pigs stimulated production of anti-peptide antibodies, which were shown to act as anti-sperm antibodies. Potentially, such anti-sperm antibodies could interfere with sperm delivery or function in the male or female genital tract, leading to contraceptive effects. Staining of semen samples collected from different mammalian species, including pig, cat, dog, bull, and mouse, with anti-sera from pigs immunized with ZP-binding phage allowed identification of phage-peptide constructs with different levels of species specificity. Based on the intensity of the immune responses and specificity of these responses in different species, two of the antigens with fusion peptide sequences GEGGYGSHD and GQQGLNGDS were recognized as the most promising candidates for development of contraceptive vaccines for wild pigs.

► ZP-binding phage with species-specific properties were identified by novel approach. ► Identified ZP-binding phage stimulated production of specific antibodies in pigs. ► Antibodies to ZP-binding phage were shown to bind sperm in a species-specific way. ► Identified ZP-binding phage have contraceptive potentials.

Keywords
ZP, zona pellucida; PZP, porcine zona pellucida; GnRH, gonadotrophin releasing hormone; cfu, colony forming unit; vir, virionZona pellucida; Recombinant filamentous phage; Anti-sperm antibody
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Phage display allows identification of zona pellucida-binding peptides with species-specific properties: Novel approach for development of contraceptive vaccines for wildlife
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issues 2–3, 31 December 2012, Pages 311–318
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us