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Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution

Paper ID Volume ID Publish Year Pages File Format Full-Text
23632 43459 2012 8 PDF Available
Title
Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution
Abstract

Microbial fermentation of intracellular product is usually limited by high intracellular product concentration inhibition and complex downstream product processing. Perstractive fermentation of intracellular Monascus pigments in the nonionic surfactant Triton X-100 aqueous solution was studied in the present work, in which the intracellular product was exported from the intracellular to the extracellular aqueous solution and consecutively extracted into the nonionic surfactant micelles. After the second stage perstractive fermentation in the two-stage operation mode, biomass increased from 5 to 24 g/l DCW. The corresponding extracellular concentrations of yellow, orange, and red pigments were 60, 49 and 26 AU. The increase of cell density and the final pigment concentration were difficult to occur in a conventional aqueous medium using the two-stage fermentation. This positive effect of perstractive fermentation was ascribed to low intracellular pigment density, which eliminated the product inhibition and prevented the product from further degradation. The high efficiency of perstractive fermentation was further confirmed by fed-batch operation mode, in which the final biomass reached 28 g/l DCW and the corresponding extracellular concentrations of yellow, orange, and red pigments were 130, 84 and 47 AU.

► Perstractive fermentation of intracellular Monascus pigments was carried out in nonionic surfactant micelle aqueous solution. ► The secretion of intracellular pigments into the extracellular broth eliminated the product inhibition. ► High extracellular pigment concentration was achieved using fed-batch operation mode.

Keywords
Perstractive fermentation; Nonionic surfactants; Permeability; Monascus pigments; Product inhibition
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Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issues 2–3, 31 December 2012, Pages 202–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us