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Treatment of Phaeodactylum tricornutum cells with papain facilitates lipid extraction

Paper ID Volume ID Publish Year Pages File Format Full-Text
23649 43460 2012 10 PDF Available
Title
Treatment of Phaeodactylum tricornutum cells with papain facilitates lipid extraction
Abstract

Triacylglycerols (TAGs) from microalgae have the potential to be used for biodiesel, but several technical and economic hurdles have to be overcome. A major challenge is efficient extraction of intracellular TAGs from algae. Here we investigate the use of enzymes to deconstruct algal cell walls/membranes. We describe a rapid and simple assay that can assess the efficacy of different enzyme treatments on TAG-containing algae. By this means crude papain and bromelain were found to be effective in releasing TAGs from the diatom Phaeodactylum tricornutum, most likely because of their cysteine protease activity. Pre-treating algal biomass with crude papain enabled complete extraction of TAGs using heptane/isopropyl alcohol. Heptane as a single solvent was also effective, although complete recovery of TAG was not obtained. Economic implications of these findings are discussed, with the aim to reduce the complexity of, and energy needed in, TAG extraction.

► Enzymes facilitated extraction of triacylglycerols (TAGs) from algae. ► Developed a rapid screening method that allowed quantification of TAG release. ► Candidate enzymes were found for three TAG-producing microalgal species. ► Papain was effective for the diatom Phaeodactylum tricornutum. ► Enzyme treatment may enable milder solvent extraction methods.

Keywords
dpi, days post inoculation; EHS, environmental-health and safety; GWP, global warming potential; IPA, isopropyl alcohol; PBS, phosphate-buffered saline; SD, standard deviation; TAG, triacylglycerol; TEM, transmission electron microscopeTriacylglycerol; Mi
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Treatment of Phaeodactylum tricornutum cells with papain facilitates lipid extraction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issue 1, 30 November 2012, Pages 40–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us