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The search for new chlorophyll-binding proteins in the cyanobacterium Synechocystis sp. PCC 6803

Paper ID Volume ID Publish Year Pages File Format Full-Text
23659 43460 2012 10 PDF Available
Title
The search for new chlorophyll-binding proteins in the cyanobacterium Synechocystis sp. PCC 6803
Abstract

Light harvesting provides a major challenge in the production of biofuels from microorganisms; while sunlight provides the energy necessary for biomass/biofuel production, at the same time it damages the cells. The genome of Synechocystis sp. PCC 6803 was searched for open reading frames that might code for yet unidentified chlorophyll-binding proteins with low molecular mass that could be involved in stress-adaptation. Amongst 9167 hypothetical ORFs corresponding to potential polypeptides of 100 amino acids or less, two were identified that had the potential to be pigment-binding, because they (i) encoded a potential transmembrane region, (ii) showed sequence similarity with known chlorophyll-binding domains, (iii) were conserved in other cyanobacterial species, and (iv) their codon adaptation index indicated significant translation probability. The two ORFs were located complementary (antisense) and internal to the ferrochelatase (hemH) and the pyruvate dehydrogenase (pdh) genes and therefore were named a-fch and a-pdh, respectively. Transcription of both genes was confirmed; however, no translated proteins could be detected immunologically. Whereas mutations within a-pdh or a-fch did not lead to any obvious phenotype, it is clear that transcripts and proteins over and above the currently known set may play a role in defining the physiology of cyanobacteria and other organisms.

► In the genome of the cyanobacterium Synechocystis sp. PCC 6803 open reading frames below 50 codons were not annotated. ► We have searched the genome for small hypothetical chlorophyll binding proteins. ► Two open reading frames, located antisense to known genes, fulfilled the criteria. ► Transcription and differential regulation of these anti-sense genes was investigated. ► While both genes are transcribed, their translation still is unclear.

Keywords
CAB, chlorophyll a/b-binding; CAI, codon adaptation index; CT, cycle threshold; LHC, light harvesting complex; Lil, light-harvesting like; ORF, open reading frame; PS, photosystem; WCE, whole cell extractAntenna; Chlorophyll-binding protein; Light-harvest
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The search for new chlorophyll-binding proteins in the cyanobacterium Synechocystis sp. PCC 6803
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issue 1, 30 November 2012, Pages 124–133
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us