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Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
2366 109 2009 11 PDF Available
Title
Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering
Abstract

Ca-deficient hydroxyapatite (CDHA) porous scaffolds were successfully fabricated from calcium phosphate cement (CPC) by a particle-leaching method. The morphology, porosity and mechanical strength as well as degradation of the scaffolds were characterized. The results showed that the CDHA scaffolds with a porosity of 81% showed open macropores with pore sizes of 400–500 μm. Thirty-six per cent of these CDHA scaffolds were degraded after 12 weeks in Tris–HCl solution. Mesenchymal stem cells (MSCs) were cultured, expanded and seeded on the scaffolds, and the proliferation and differentiation of MSCs into osteoblastic phenotype were determined using MTT assay, alkaline phosphatase activity and scanning electron microscopy. The results revealed that the CDHA scaffolds were biocompatible and had no negative effects on the MSCs in vitro. The in vivo biocompatibility and osteogenicity of the scaffolds were investigated. Both CDHA scaffolds and MSC/scaffold constructs were implanted in rabbit mandibles and studied histologically. The results showed that CDHA scaffolds exhibited good biocompatibility and osteoconductivity. Moreover, the introduction of MSCs into the scaffolds dramatically enhanced the efficiency of new bone formation, especially at the initial stage after implantation (from 2 to 4 weeks). However, the CDHA scaffolds showed as good biocompatibility and osteogenicity as the hybrid ones at 8 weeks. These results indicate that the CDHA scaffolds fulfill the basic requirements of bone tissue engineering scaffold.

Keywords
Ca-deficient hydroxyapatite scaffold; Mesenchymal stem cells; Biocompatibility; Osteogenesis; Bone tissue engineering
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Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 1, January 2009, Pages 268–278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us