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On the dynamics and constraints of batch culture growth of the cyanobacterium Cyanothece sp. ATCC 51142

Paper ID Volume ID Publish Year Pages File Format Full-Text
23661 43460 2012 8 PDF Available
Title
On the dynamics and constraints of batch culture growth of the cyanobacterium Cyanothece sp. ATCC 51142
Abstract

The unicellular, nitrogen fixing cyanobacterium Cyanothece sp. ATCC 51142 is of a remarkable potential for production of third-generation biofuels. As the biotechnological potential of Cyanothece 51142 varies with the time of the day, we argue that it will, similarly, depend on the phase of the culture growth. Here, we study the batch culture dynamics to discover the dominant constraints in the individual growth phases and identify potential for inducing or delaying transitions between culture growth phases in Cyanothece 51142. We found that specific growth rate in the exponential phase of the culture is much less dependent on incident irradiance than the photosynthetic activity. We propose that surplus electrons that are released by water splitting are used in futile processes providing photoprotection additional to non-photochemical quenching. We confirm that the transition from exponential to linear phase is caused by a light limitation and the transition from linear to stationary phase by nitrogen limitation. We observe spontaneous diurnal metabolic oscillations in stationary phase culture that are synchronized over the entire culture without an external clue. We tentatively propose that the self-synchronization of the metabolic oscillations is due to a cell-to-cell communication of the cyanobacteria that is necessary for nitrogenase activity in nitrate depleted medium.

► Growth is saturated by light much earlier than the photosynthetic activity. ► Photoprotection by futile electron transport can be responsible for the difference. ► Spontaneous diurnal oscillations occur with nitrate deficiency. ► Oscillatory self-synchronization may be due to a cell-to-cell communication.

Keywords
Ft, steady state fluorescence emission yield; LED, light emitting diodes; PAR, photosynthetically active radiation; TAPS, N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid sodium-potassium salt; TAPSO, 2-hydroxy-3-[tris(hydroxymethyl)methylamino]-1-
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On the dynamics and constraints of batch culture growth of the cyanobacterium Cyanothece sp. ATCC 51142
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 162, Issue 1, 30 November 2012, Pages 148–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us