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Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae

Paper ID Volume ID Publish Year Pages File Format Full-Text
23666 43461 2012 9 PDF Available
Title
Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae
Abstract

In the present study an arsenite, As(III), tolerating bacterium, MR4, was isolated from Mulla River Pune, India, capable of reducing arsenate to arsenite and identified as Klebsiella pneumoniae (HQ857583). Comparative proteomic analysis using two-dimensional gel electrophoresis (2-DGE) and matrix assisted laser desorption ionization-time of flight-time of flight (MALDI-TOF/TOF) was used to monitor the proteins undergoing changes in expression levels under 2.5 mM As(III) stress. The 2-DGE proteome map has shown that 60 proteins were differentially expressed under As(III) stress, of which 39 proteins were successfully identified with a MASCOT score greater than 70 (p < 0.05). Among the identified proteins, membrane transport/binding proteins, porins, and amino acid metabolism enzymes were down-regulated while stress responsive proteins and antioxidant enzymes were up-regulated. Proteins involved in carbohydrate metabolism, particularly those in pentose phosphate pathway were also up-regulated while those involved in pyruvate metabolism were down-regulated. However, proteins involved in glycolysis and tricarboxylic acid cycle showed a mixed regulation response. These findings provide new insights into the probable mechanisms by which K. pneumoniae (HQ857583) could be adapting to As(III) stress.

► We examine changes in the expression levels of proteins under As(III) stress. ► K. pneumoniae (HQ857583) is arsenate reducing bacteria which converts As(V) to As(III). ► Down-regulation of porins and hexose permease increases As(III) resistancy. ► Up-regulated antioxidant enzymes and stress responsive proteins protect the cell during As(III) stress.

Keywords
CHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; DTT, dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI-TOF-TOF, matrix assisted laser desorption ionization-time of flight-time of flightKlebsiella pneumoniae;
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Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 158, Issues 1–2, 31 March 2012, Pages 8–16
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us