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Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia

Paper ID Volume ID Publish Year Pages File Format Full-Text
23667 43461 2012 7 PDF Available
Title
Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia
Abstract

A putative fatty acid hydratase from Stenotrophomonas maltophilia was cloned and expressed in Escherichia coli. The recombinant enzyme showed the highest hydration activity for oleic acid among the fatty acids tested, indicating that the enzyme is an oleate hydratase. The optimal conditions for the production of 10-hydroxystearic acid from oleic acid using whole cells of recombinant E. coli containing the oleate hydratase were pH 6.5, 35 °C, 0.05% (w/v) Tween 40, 10 g l−1 cells, and 50 g l−1 oleic acid. Under these conditions, whole recombinant cells produced 49 g l−1 10-hydroxystearic acid for 4 h, with a conversion yield of 98% (w/w), a volumetric productivity of 12.3 g l−1 h−1, and a specific productivity of 1.23 g g-cells−1 h−1, which were 18%, 2.5-, and 2.5-fold higher than those of whole wild-type S. maltophilia cells, respectively. This is the first report of 10-hydroxystearic acid production using recombinant cells and the concentration and productivity are the highest reported thus far among cells.

► Oleate hydratase from Stenotrophomonas maltophilia is characterized. ► Recombinant E. coli containing the oleate hydratase produced 10-hydroxystearic acid. ► This is the first report of 10-hydroxystearic acid production by recombinant cells. ► The concentration and productivity of the product are the highest reported thus far.

Keywords
Stenotrophomonas maltophilia; Oleic acid; 10-Hydroxystearic acid production; Oleate hydratase; Recombinant Escherichia coli
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Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 158, Issues 1–2, 31 March 2012, Pages 17–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us