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Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphATe gene expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
23672 43461 2012 9 PDF Available
Title
Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphATe gene expression
Abstract

A chimeric cyanophycin synthetase gene composed of the cphATe coding region from the cyanobacterium Thermosynechococcus elongatus BP-1, the constitutive 35S promoter and the plastid targeting sequence of the integral photosystem II protein PsbY was transferred to the tobacco variety Petit Havanna SRI and the commercial potato starch production variety Albatros. The resulting constitutive expression of cyanophycin synthetase leads to polymer contents in potato leaf chloroplasts of up to 35 mg/g dry weight and in tuber amyloplasts of up to 9 mg/g dry weight. Both transgenic tobacco and potato were used for the development of isolation methods applicable for large-scale extraction of the polymer. Two different procedures were developed which yielded polymer samples of 80 and 90% purity, respectively

► A chimeric cyanophycin synthetase gene was transferred to the commercial potato starch production variety Albatros. ► Cyanophycin contents were in potato leaf chloroplasts of up to 35 mg/g dry weight and in tuber amyloplasts of up to 9 mg/g dry weight. ► Transgenic plants were used for the development of isolation methods applicable for large-scale extraction of the polymer. ► Two different procedures were developed which yielded polymer samples of 80 and 90% purity, respectively.

Keywords
Biopolymer; Cyanophycin; Polyaspartic acid; Transgenic plants; Tobacco; Potato
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Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphATe gene expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 158, Issues 1–2, 31 March 2012, Pages 50–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us