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Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
23673 43461 2012 9 PDF Available
Title
Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression
Abstract

Amaranth seeds are considered as an excellent complementary source of food protein due to their balanced amino acid composition. Amarantin acidic subunit has the potential as a functional and nutraceutical protein, and it is structurally a good candidate for modification. The aim of this work was to improve its functionality, then the primary structure was modified into the third variable region of 11S globulins, by inserting antihypertensive peptides: four Val-Tyr in tandem and Arg-Ile-Pro-Pro in the C-terminal region. Modified protein was expressed in Escherichia coli Origami (DE3) and was purified. The culture conditions, including the culture media, temperature, agitation speed and air flow were tested in order to obtain an increased expression levels of the modified protein. A 23 factorial design was used for evaluate the effect of environmental conditions on modified protein production. The results indicated that the yield of modified protein could be increased by up 3-fold in bioreactor as compared with flask. In addition, the temperature, the agitation speed and the oxygen were significant factors on the expression of the antihypertensive protein. The maximum production was 99 mg protein-L−1. The hydrolyzed protein showed a high inhibitory activity of the angiotensin converting enzyme (IC50 = 0.047 mg mL−1).

► We inserted 4 VY and 1 IPP antihypertensive peptides in the amarantin acidic subunit. ► Modified protein was overexpressed in Escherichia coli in a fermentor finding the best factors. ► The temperature, agitation speed and oxygen had effect on the yield of the protein. ► Modified protein is ten times more active than the non-modified sample.

Keywords
Amaranth seed; Antihypertensive peptides; 11S acidic subunit; ACE inhibitory activity
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Overexpression of a modified protein from amaranth seed in Escherichia coli and effect of environmental conditions on the protein expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 158, Issues 1–2, 31 March 2012, Pages 59–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us