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Evaluation of transgenic tobacco plants expressing a bacterial Co–Ni transporter for acquisition of cobalt

Paper ID Volume ID Publish Year Pages File Format Full-Text
23676 43462 2012 7 PDF Available
Title
Evaluation of transgenic tobacco plants expressing a bacterial Co–Ni transporter for acquisition of cobalt
Abstract

Phytoremediation is a viable strategy for management of toxic wastes in a large area/volume with low concentrations of toxic elemental pollutants. With increased industrial use of cobalt and its alloys, it has become a major metal contaminant in soils and water bodies surrounding these industries and mining sites with adverse effects on the biota. A bacterial Co–Ni permease was cloned from Rhodopseudomonas palustris and introduced into Nicotiana tabacum to explore its potential for phytoremediation and was found to be specific for cobalt and nickel. The transgenic plants accumulated more cobalt and nickel as compared to control, whereas no significant difference in accumulation of other divalent ions was observed. The transgenic plants were evaluated for cobalt content and showed increased acquisition of cobalt (up to 5 times) as compared to control. The plants were also assessed for accumulation of nickel and found to accumulate up to 2 times more nickel than control. At the same initial concentration of cobalt and nickel, transgenic plant preferentially accumulated cobalt as compared to nickel. The present study is perhaps the first attempt to develop transgenic plants expressing heterologous Co transporter with an improved capacity to uptake cobalt.

► A Ni–Co permease from Rhodopseudomonas palustris was expressed in tobacco. ► Transgenic plants showed significantly increased accumulation specific to Co and Ni. ► At same initial concentration a preferential uptake of Co over Ni was observed. ► Most of the Co accumulated in root with limited translocation to shoots. ► These transgenic plants can find application for phytostabilization.

Keywords
Transgenic tobacco; Phytoremediation; Ni–Co permeases; Cobalt; Nickel
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Evaluation of transgenic tobacco plants expressing a bacterial Co–Ni transporter for acquisition of cobalt
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 161, Issue 4, 15 November 2012, Pages 422–428
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us