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Thermorecovery of cyanobacterial fatty acids at elevated temperatures

Paper ID Volume ID Publish Year Pages File Format Full-Text
23679 43462 2012 5 PDF Available
Title
Thermorecovery of cyanobacterial fatty acids at elevated temperatures
Abstract

We have developed a genetic system we call “thermorecovery” that allows us to lyse cyanobacterial cultures and hydrolyze membrane lipids to release free fatty acids (FFAs), a biofuel precursor. The system uses thermostable lipases encoded by genes from thermophilic organisms that have been transferred into the cyanobacterial genome and can be synthesized by turning off CO2 availability and subsequently activated by increasing the concentrated culture temperature. When synthesized in FFA-producing strains, the lipase Fnl from Fervidobacterium nodosum Rt17-B1 released the most FFA. Of the seven candidate lipases investigated, Fnl-synthesizing strains yielded 42.7 ± 1.5 mg/l FFA at 47 °C. We also determined that the optimal production conditions for SD338, the Synechocystis strain synthesizing Fnl, was to keep the cell concentrates at 46°C for two days after a one-day CO2 limitation pretreatment of the culture. A 4-l continuous semi-batch production experiment with SD338 showed that daily harvested cultures (1 l) released an average of 43.9 ± 6.6 mg fatty acid and this productivity lasted for at least 20 days without significant decline. This improved thermorecovery process can be used in conjunction with other means to genetically engineer cyanobacteria to produce biofuels or biofuel precursors as the final step in recovery of membrane lipids.

► Thermorecovery can produce biofuel precursors from engineered cyanobacteria. ► Thermostable lipase genes were transferred into the cyanobacterial genome. ► The lipases were activated by increasing the temperature of the culture. ► Cell membrane lipids were hydrolyzed into fatty acids at 47 °C. ► Fatty acid recovery can occur in concentrated cultures without light.

Keywords
Cyanobacteria; Fatty acid; Thermostable lipases; Biofuel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 161, Issue 4, 15 November 2012, Pages 445–449
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us