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Influence of structured wafer surfaces on the characteristics of Caco-2 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
2368 109 2009 10 PDF Available
Title
Influence of structured wafer surfaces on the characteristics of Caco-2 cells
Abstract

Currently there is an increasing demand for high-throughput methods to identify and to verify the potential of new drug candidates. Cell-based microelectronic biosensors might be powerful tools for rapid screening assays. However, reliable cultivation of cells is influenced by the material characteristics and the surface topography of the wafers serving as growth supports. In order to investigate the influence of micropatterned structures on cell viability, Caco-2 cells were seeded on silicon wafers featuring trench/mesa patterns obtained by lithography and reactive ion etching. Besides determination of the cell growth pattern by electron microscopic inspection, the adherence of cells on different patterned silicon wafers and the formation of the tight junctional network was investigated. Microstructured trench/mesa patterns, especially their lateral distances, remarkably influenced the adhesion and proliferation behavior of Caco-2 cells. Lateral distances below the average cell diameter were easily overgrown by the cells, whereas dimensions above the average cell diameter increasingly limited cell proliferation. Notably increased cell growth was observed using trenches with a width of 10–20 μm and a trench depth of around 35 μm. All in all, the results of this study might improve the production of microstructured biosensors and open up new perspectives concerning the combination of biosensors and microfluidic systems.

Keywords
Caco-2; Structured surfaces; Cell adhesion; Trench; Mesa
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 5, Issue 1, January 2009, Pages 288–297
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us