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An automated multiplex specific IgE assay system using a photoimmobilized microarray

Paper ID Volume ID Publish Year Pages File Format Full-Text
23681 43462 2012 8 PDF Available
Title
An automated multiplex specific IgE assay system using a photoimmobilized microarray
Abstract

An automated microarray diagnostic system for specific IgE using photoimmobilized allergen has been developed. Photoimmobilization is useful for preparing microarrays, where various types of biological components are covalently immobilized on a plate. Because the immobilization is based on a photo-induced radical cross-linking reaction, it does not require specific functional groups on the immobilized components. Here, an aqueous solution of a photoreactive poly(ethylene glycol)-based polymer was spin-coated on a plate, and an aqueous solution of each allergen was microspotted on the coated plate and allowed to dry in air. Finally, the plate was irradiated with an ultraviolet lamp for covalent immobilization. An automated machine using these plates was developed for the assay of antigen-specific IgE. Initially, the patient serum was added to the microarray plate, and after reaction of the microspotted allergen with IgE, the adsorbed IgE was detected by a peroxidase-conjugated anti-IgE-antibody. The chemical luminescence intensity of the substrate decomposed by the peroxidase was automatically detected using a sensitive charge-coupled device camera. All the allergens were immobilized stably using this method, which was used to screen for allergen-specific IgE. The results were comparable with those using conventional specific IgE. Using this system, six different allergen-specific IgE were assayed using 10 μL of serum within a period of 20 min.

► An automated microarray diagnostic system for specific IgE was developed. ► Photoimmobilization was employed for preparing the microarrays. ► All the allergens were immobilized stably and used to screen for allergen-specific IgE. ► Six allergen-specific IgEs were assayed using 10 μL of serum during 20 min.

Keywords
Microarray; Biochip; Automated assay; Photoimmobilization
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An automated multiplex specific IgE assay system using a photoimmobilized microarray
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 161, Issue 4, 15 November 2012, Pages 414–421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us